Microstructural and electrochemical impedance characterization of bio-functionalized ultrafine ZnS nanocrystals-reduced graphene oxide hybrid for immunosensor applications.
Identifieur interne : 000547 ( Main/Exploration ); précédent : 000546; suivant : 000548Microstructural and electrochemical impedance characterization of bio-functionalized ultrafine ZnS nanocrystals-reduced graphene oxide hybrid for immunosensor applications.
Auteurs : RBID : pubmed:24056976English descriptors
- KwdEn :
- Antibodies (immunology), Biosensing Techniques, Carbodiimides (chemistry), Dielectric Spectroscopy, Electrochemical Techniques, Electrodes, Graphite (chemistry), Humans, Myoglobin (analysis), Myoglobin (immunology), Oxides (chemistry), Quantum Dots (chemistry), Sulfides (chemistry), Tin Compounds (chemistry), Zinc Compounds (chemistry).
- MESH :
- chemical , analysis : Myoglobin.
- chemical , chemistry : Carbodiimides, Graphite, Oxides, Sulfides, Tin Compounds, Zinc Compounds.
- chemical , immunology : Antibodies, Myoglobin.
- chemistry : Quantum Dots.
- Biosensing Techniques, Dielectric Spectroscopy, Electrochemical Techniques, Electrodes, Humans.
Abstract
We report a mercaptopropionic acid capped ZnS nanocrystals decorated reduced graphene oxide (RGO) hybrid film on a silane modified indium-tin-oxide glass plate, as a bioelectrode for the quantitative detection of human cardiac myoglobin (Ag-cMb). The ZnS nanocrystals were anchored over electrochemically reduced GO sheets through a cross linker, 1-pyrenemethylamine hydrochloride, by carbodiimide reaction and have been characterized by scanning electron microscopy, transmission electron microscopy and energy dispersive X-ray spectroscopy. The transmission electron microscopic characterization of the ZnS-RGO hybrid shows the uniform distribution of ultra-fine nanoparticles of ZnS in nano-sheets of GO throughout the material. The protein antibody, Ab-cMb, was covalently linked to ZnS-RGO nanocomposite hybrid for the fabrication of the bioelectrode. A detailed electrochemical immunosensing study has been carried out on the bioelectrode towards the detection of target Ag-cMb. The optimal fitted equivalent circuit model that matches the impedance response has been studied to delineate the biocompatibility, sensitivity and selectivity of the bioelectrode. The bioelectrode exhibited a linear electrochemical impedance response to Ag-cMb in a range of 10 ng to 1 μg mL(-1) in PBS (pH 7.4) with a sensitivity of 177.56 Ω cm(2) per decade. The combined synergistic effects of the high surface-to-volume ratio of ZnS(MPA) nanocrystals and conducting RGO has provided a dominant charge transfer characteristic (R(et)) at the lower frequency region of <10 Hz showing a good biocompatibility and enhanced impedance sensitivity towards target Ag-cMb. The impedance response sensitivity of the ZnS-RGO hybrid bioelectrode towards Ag-cMb has been found to be about 2.5 fold higher than that of a bare RGO modified bioelectrode.
DOI: 10.1039/c3nr02575f
PubMed: 24056976
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Microstructural and electrochemical impedance characterization of bio-functionalized ultrafine ZnS nanocrystals-reduced graphene oxide hybrid for immunosensor applications.</title><author><name sortKey="Mishra, Sujeet K" uniqKey="Mishra S">Sujeet K Mishra</name><affiliation wicri:level="1"><nlm:affiliation>CSIR-National Physical Laboratory, Dr K. S. Krishnan Road, New Delhi, 110012, India. rajesh_csir@yahoo.com.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>CSIR-National Physical Laboratory, Dr K. S. Krishnan Road, New Delhi, 110012</wicri:regionArea></affiliation></author><author><name sortKey="Srivastava, Avanish K" uniqKey="Srivastava A">Avanish K Srivastava</name></author><author><name sortKey="Kumar, Devendra" uniqKey="Kumar D">Devendra Kumar</name></author><author><name sortKey="Biradar, Ashok M" uniqKey="Biradar A">Ashok M Biradar</name></author><author><name sortKey="Rajesh" uniqKey="Rajesh">Rajesh</name></author></titleStmt><publicationStmt><date when="2013">2013</date><idno type="doi">10.1039/c3nr02575f</idno><idno type="RBID">pubmed:24056976</idno><idno type="pmid">24056976</idno><idno type="wicri:Area/Main/Corpus">000396</idno><idno type="wicri:Area/Main/Curation">000396</idno><idno type="wicri:Area/Main/Exploration">000547</idno></publicationStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antibodies (immunology)</term><term>Biosensing Techniques</term><term>Carbodiimides (chemistry)</term><term>Dielectric Spectroscopy</term><term>Electrochemical Techniques</term><term>Electrodes</term><term>Graphite (chemistry)</term><term>Humans</term><term>Myoglobin (analysis)</term><term>Myoglobin (immunology)</term><term>Oxides (chemistry)</term><term>Quantum Dots (chemistry)</term><term>Sulfides (chemistry)</term><term>Tin Compounds (chemistry)</term><term>Zinc Compounds (chemistry)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Myoglobin</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Carbodiimides</term><term>Graphite</term><term>Oxides</term><term>Sulfides</term><term>Tin Compounds</term><term>Zinc Compounds</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies</term><term>Myoglobin</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Quantum Dots</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Biosensing Techniques</term><term>Dielectric Spectroscopy</term><term>Electrochemical Techniques</term><term>Electrodes</term><term>Humans</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We report a mercaptopropionic acid capped ZnS nanocrystals decorated reduced graphene oxide (RGO) hybrid film on a silane modified indium-tin-oxide glass plate, as a bioelectrode for the quantitative detection of human cardiac myoglobin (Ag-cMb). The ZnS nanocrystals were anchored over electrochemically reduced GO sheets through a cross linker, 1-pyrenemethylamine hydrochloride, by carbodiimide reaction and have been characterized by scanning electron microscopy, transmission electron microscopy and energy dispersive X-ray spectroscopy. The transmission electron microscopic characterization of the ZnS-RGO hybrid shows the uniform distribution of ultra-fine nanoparticles of ZnS in nano-sheets of GO throughout the material. The protein antibody, Ab-cMb, was covalently linked to ZnS-RGO nanocomposite hybrid for the fabrication of the bioelectrode. A detailed electrochemical immunosensing study has been carried out on the bioelectrode towards the detection of target Ag-cMb. The optimal fitted equivalent circuit model that matches the impedance response has been studied to delineate the biocompatibility, sensitivity and selectivity of the bioelectrode. The bioelectrode exhibited a linear electrochemical impedance response to Ag-cMb in a range of 10 ng to 1 μg mL(-1) in PBS (pH 7.4) with a sensitivity of 177.56 Ω cm(2) per decade. The combined synergistic effects of the high surface-to-volume ratio of ZnS(MPA) nanocrystals and conducting RGO has provided a dominant charge transfer characteristic (R(et)) at the lower frequency region of <10 Hz showing a good biocompatibility and enhanced impedance sensitivity towards target Ag-cMb. The impedance response sensitivity of the ZnS-RGO hybrid bioelectrode towards Ag-cMb has been found to be about 2.5 fold higher than that of a bare RGO modified bioelectrode.</div></front></TEI><pubmed><MedlineCitation Owner="NLM" Status="MEDLINE"><PMID Version="1">24056976</PMID><DateCreated><Year>2013</Year><Month>10</Month><Day>11</Day></DateCreated><DateCompleted><Year>2014</Year><Month>05</Month><Day>14</Day></DateCompleted><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">2040-3372</ISSN><JournalIssue CitedMedium="Internet"><Volume>5</Volume><Issue>21</Issue><PubDate><Year>2013</Year><Month>Nov</Month><Day>7</Day></PubDate></JournalIssue><Title>Nanoscale</Title><ISOAbbreviation>Nanoscale</ISOAbbreviation></Journal><ArticleTitle>Microstructural and electrochemical impedance characterization of bio-functionalized ultrafine ZnS nanocrystals-reduced graphene oxide hybrid for immunosensor applications.</ArticleTitle><Pagination><MedlinePgn>10494-503</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1039/c3nr02575f</ELocationID><Abstract><AbstractText>We report a mercaptopropionic acid capped ZnS nanocrystals decorated reduced graphene oxide (RGO) hybrid film on a silane modified indium-tin-oxide glass plate, as a bioelectrode for the quantitative detection of human cardiac myoglobin (Ag-cMb). The ZnS nanocrystals were anchored over electrochemically reduced GO sheets through a cross linker, 1-pyrenemethylamine hydrochloride, by carbodiimide reaction and have been characterized by scanning electron microscopy, transmission electron microscopy and energy dispersive X-ray spectroscopy. The transmission electron microscopic characterization of the ZnS-RGO hybrid shows the uniform distribution of ultra-fine nanoparticles of ZnS in nano-sheets of GO throughout the material. The protein antibody, Ab-cMb, was covalently linked to ZnS-RGO nanocomposite hybrid for the fabrication of the bioelectrode. A detailed electrochemical immunosensing study has been carried out on the bioelectrode towards the detection of target Ag-cMb. The optimal fitted equivalent circuit model that matches the impedance response has been studied to delineate the biocompatibility, sensitivity and selectivity of the bioelectrode. The bioelectrode exhibited a linear electrochemical impedance response to Ag-cMb in a range of 10 ng to 1 μg mL(-1) in PBS (pH 7.4) with a sensitivity of 177.56 Ω cm(2) per decade. The combined synergistic effects of the high surface-to-volume ratio of ZnS(MPA) nanocrystals and conducting RGO has provided a dominant charge transfer characteristic (R(et)) at the lower frequency region of <10 Hz showing a good biocompatibility and enhanced impedance sensitivity towards target Ag-cMb. The impedance response sensitivity of the ZnS-RGO hybrid bioelectrode towards Ag-cMb has been found to be about 2.5 fold higher than that of a bare RGO modified bioelectrode.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Mishra</LastName><ForeName>Sujeet K</ForeName><Initials>SK</Initials><Affiliation>CSIR-National Physical Laboratory, Dr K. S. Krishnan Road, New Delhi, 110012, India. rajesh_csir@yahoo.com.</Affiliation></Author><Author ValidYN="Y"><LastName>Srivastava</LastName><ForeName>Avanish K</ForeName><Initials>AK</Initials></Author><Author ValidYN="Y"><LastName>Kumar</LastName><ForeName>Devendra</ForeName><Initials>D</Initials></Author><Author ValidYN="Y"><LastName>Biradar</LastName><ForeName>Ashok M</ForeName><Initials>AM</Initials></Author><Author ValidYN="Y"><LastName>Rajesh</LastName></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType>Journal Article</PublicationType><PublicationType>Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2013</Year><Month>09</Month><Day>17</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Nanoscale</MedlineTA><NlmUniqueID>101525249</NlmUniqueID><ISSNLinking>2040-3364</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Antibodies</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Carbodiimides</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Myoglobin</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Oxides</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Sulfides</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Tin Compounds</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Zinc Compounds</NameOfSubstance></Chemical><Chemical><RegistryNumber>71243-84-0</RegistryNumber><NameOfSubstance>indium tin oxide</NameOfSubstance></Chemical><Chemical><RegistryNumber>7782-42-5</RegistryNumber><NameOfSubstance>Graphite</NameOfSubstance></Chemical><Chemical><RegistryNumber>KPS085631O</RegistryNumber><NameOfSubstance>zinc sulfide</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName MajorTopicYN="N">Antibodies</DescriptorName><QualifierName MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="Y">Biosensing Techniques</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Carbodiimides</DescriptorName><QualifierName MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Dielectric Spectroscopy</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="Y">Electrochemical Techniques</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Electrodes</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Graphite</DescriptorName><QualifierName MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Myoglobin</DescriptorName><QualifierName MajorTopicYN="Y">analysis</QualifierName><QualifierName MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Oxides</DescriptorName><QualifierName MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Quantum Dots</DescriptorName><QualifierName MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Sulfides</DescriptorName><QualifierName MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Tin Compounds</DescriptorName><QualifierName MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Zinc Compounds</DescriptorName><QualifierName MajorTopicYN="Y">chemistry</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="aheadofprint"><Year>2013</Year><Month>9</Month><Day>17</Day></PubMedPubDate><PubMedPubDate PubStatus="epublish"><Year>2013</Year><Month>10</Month><Day>10</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2013</Year><Month>9</Month><Day>24</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2013</Year><Month>9</Month><Day>24</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2014</Year><Month>5</Month><Day>16</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="doi">10.1039/c3nr02575f</ArticleId><ArticleId IdType="pubmed">24056976</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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